From the single coral fragment had been collected from every exposure issue
From the solitary coral fragment were gathered from each exposure issue and stuck in Carnoy’s answer (60 ethanol, thirty chloroform and 10 glacial acetic acid). Methods for CaCO3 skeleton demineralization, tissue embedding, histological tissue sectioning, histochemical staining, impression acquisition and evaluation are described in Piggot et al. . Briefly,…